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5-Cholesten-3-«beta»,27-diol, TMS


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
PackedOV-1265.3450.Gaskell, Smith, et al., 1975N2; Column length: 2. m

Kovats' RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryMethyl Silicone3470.Shoda, Axelson, et al., 199325. m/0.32 mm/0.25 «mu»m, 30. K/min; Tstart: 60. C; Tend: 290. C

Normal alkane RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryMethyl Silicone3459.Ichimiya, Egestad, et al., 199125. m/0.32 mm/0.25 «mu»m, He, 25. K/min; Tstart: 80. C; Tend: 280. C

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryUltra-13450.Axelson, Ellis, et al., 200025. m/0.32 mm/0.17 «mu»m, H2; Program: 50 0C (3 min) -> 185 0C 5 K/min -> 280 0C
CapillaryUltra-13455.Axelson and Larsson, 199625. m/0.32 mm/0.17 «mu»m; Program: 50C (3min) => (rapidly) => 185C => 5C/min => 280C

References

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Gaskell, Smith, et al., 1975
Gaskell, S.J.; Smith, A.G.; Brooks, C.J.W., Gas chromatography mass spectrometry of trimethylsilyl ethers of sidechain hydroxylated «DELTA»4-3-ketosteroids. Long range trimethylsilyl group migration under electron impact, Biomed. Mass Spectrom., 1975, 2, 3, 148-155, https://doi.org/10.1002/bms.1200020309 . [all data]

Shoda, Axelson, et al., 1993
Shoda, J.; Axelson, M.; Sjövall, J., Synthesis of potential C27-intermediates in bile acid biosynthesis and their deuterium-labeled analogs, Steroids, 1993, 58, 3, 119-125, https://doi.org/10.1016/0039-128X(93)90048-R . [all data]

Ichimiya, Egestad, et al., 1991
Ichimiya, H.; Egestad, B.; Nazer, H.; Baginski, E.S.; Clayton, P.T.; Sjövall, J., Bile acids and bile alcohols in a child with hepatic 3«beta»-hydroxy-«DELTA»5-C27-steroid dehydrogenase deficiency: effects of chenodeoxycholic acid treatment, J. Lipid Res., 1991, 32, 829-841. [all data]

Axelson, Ellis, et al., 2000
Axelson, M.; Ellis, E.; Mörk, B.; Garmark, K.; Abrahamsson, A.; Björkhem, I.; Ericzon, B.-G.; Einarsson, C., Bile acid synthesis in cultured human hepatocytes: support for an alternative biosynthetic pathway to cholic acid, Hepatology, 2000, 31, 6, 1305-1312, https://doi.org/10.1053/jhep.2000.7877 . [all data]

Axelson and Larsson, 1996
Axelson, M.; Larsson, O., 27-Hydroxylated low density lipoprotein (LDL) cholesterol can be converted to 7«alpha»,27-Dihydroxy-4-cholesten-3-one (cytosterone) before suppressing cholesterol production in normal human fibroblasts. Evidence that an altered metabolism of LDL cholesterol can underlie a defective feedback control in malignant cells, J. Biol. Chem., 1996, 271, 22, 12724-12736. [all data]


Notes

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