5-Hydroxyindoleacetic acid, 3TMS derivative
- Formula: C19H33NO3Si3
- Molecular weight: 407.7267
- IUPAC Standard InChI: InChI=1S/C19H33NO3Si3/c1-24(2,3)20-14-15(12-19(21)23-26(7,8)9)17-13-16(10-11-18(17)20)22-25(4,5)6/h10-11,13-14H,12H2,1-9H3
- IUPAC Standard InChIKey: RUKKQUFMPLELJQ-UHFFFAOYSA-N
- CAS Registry Number: 55268-67-2
- Chemical structure:
This structure is also available as a 2d Mol file or as a computed 3d SD file
The 3d structure may be viewed using Java or Javascript. - Other names: 1H-Indole-3-acetic acid, 1-(trimethylsilyl)-5-[(trimethylsilyl)oxy]-, trimethylsilyl ester; 5-Hydroxyindoleacetic acid (3TMS); 5-Hydroxyindole-3-acetic acid, tris-TMS; Indol-3-acetic acid, 5-hydroxy, (3TMS)-; Indol-3-acetic acid, 5-hydroxy, tri-TMS; Indole-3-acetic acid, 5-hydroxy, tris-TMS; Indole-3-acetic acid, 5-hydroxy, TMS; 5-Hydroxyindoleacetic acid (tms); Indol-3-acetic acid, 5-hydroxy, TMS; 5-Hydroxyindole-3-acetic acid, TMS
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Gas Chromatography
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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.
Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director
Van Den Dool and Kratz RI, non-polar column, temperature ramp
| Column type | Active phase | I | Reference | Comment |
|---|---|---|---|---|
| Capillary | DB-1 | 2199. | Hoffmann, Aramaki, et al., 1989 | 30. m/0.53 mm/1. μm, He, 4. K/min; Tstart: 70. C; Tend: 290. C |
| Capillary | SE-52 | 2239. | Lefevere, Verhaeghe, et al., 1989 | 25. m/0.32 mm/0.52 μm, He, 5. K/min; Tstart: 70. C; Tend: 270. C |
| Capillary | CP Sil 5 CB | 2168. | Wurth, Kumps, et al., 1989 | N2, 60. C @ 0.5 min, 6. K/min, 280. C @ 5. min |
| Packed | OV-1 | 2200. | Tanaka, Hine, et al., 1980 | N2, 100-120 mesh, 70. C @ 0.5 min, 28. K/min; Column length: 1.8 m; Tend: 290. C |
| Packed | OV-1 | 2204. | Butts, 1972 | He, Chromosorb W HP (80-100 mesh), 10. K/min; Column length: 2. m; Tstart: 100. C; Tend: 325. C |
Van Den Dool and Kratz RI, non-polar column, custom temperature program
| Column type | Active phase | I | Reference | Comment |
|---|---|---|---|---|
| Capillary | DB-1 | 2199. | Tsai, Oliphant, et al., 1985 | He; Program: not specified |
Normal alkane RI, non-polar column, temperature ramp
| Column type | Active phase | I | Reference | Comment |
|---|---|---|---|---|
| Capillary | HP-5MS | 2249. | Lee, Yanamandra, et al., 2002 | 30. m/0.25 mm/0.25 μm, He, 70. C @ 3. min, 4. K/min, 280. C @ 3. min |
| Packed | OV-1 | 2190. | Francis, Smith, et al., 1980 | He, 10. K/min; Column length: 1.5 m; Tstart: 180. C; Tend: 320. C |
References
Go To: Top, Gas Chromatography, Notes
Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.
Hoffmann, Aramaki, et al., 1989
Hoffmann, G.; Aramaki, S.; Blum-Hoffmann, E.; Nyhan, W.L.; Sweetman, L.,
Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis,
Clin. Chem., 1989, 35, 4, 587-595. [all data]
Lefevere, Verhaeghe, et al., 1989
Lefevere, M.F.; Verhaeghe, B.J.; Declerck, D.H.; Van Bocxlaer, J.F.; De Leenheer, A.P.; De Sagher, R.M.,
Metabolic Profiling of Urinary Organic Acids by Single and Multicolumn Capillary Gas Chromatography,
J. Chromatogr. Sci., 1989, 27, 1, 23-29, https://doi.org/10.1093/chromsci/27.1.23
. [all data]
Wurth, Kumps, et al., 1989
Wurth, C.; Kumps, A.; Mardens, Y.,
Urinary organic acids: Retention indices on two capillary gas chromatography columns,
J. Chromatogr., 1989, 491, 186-192, https://doi.org/10.1016/S0378-4347(00)82831-5
. [all data]
Tanaka, Hine, et al., 1980
Tanaka, K.; Hine, D.G.; West-Dull, A.; Lynn, T.B.,
Gas-chromatographic method of analysis for urinary organic acids. I. Retention indices of 155 metabolically important compounds,
Clin. Chem., 1980, 26, 13, 1839-1846. [all data]
Butts, 1972
Butts, W.C.,
Two-Column Gas Chromatography of Trimethylsilyl Derivatives of Biochemically Significant Compounds,
Anal. Biochemistry, 1972, 46, 1, 187-199, https://doi.org/10.1016/0003-2697(72)90411-3
. [all data]
Tsai, Oliphant, et al., 1985
Tsai, M.Y.; Oliphant, C.; Josephson, M.W.,
Identification of Metabolites Diagnostic for Organic Acidurias by Simultaneous Dual-Column Capillary Gas Chromatography,
J. Chromatogr., 1985, 341, 1-10, https://doi.org/10.1016/S0378-4347(00)84004-9
. [all data]
Lee, Yanamandra, et al., 2002
Lee, B.Y.; Yanamandra, K.; Thurmon, T.F.,
Quantitative estimation of organic analytes with a capillary column,
Am. Clin. Lab., 2002, May, 30-34. [all data]
Francis, Smith, et al., 1980
Francis, P.; Smith, I.; Finnie, M.D.A.,
Mass spectrometric and chromatographic characteristics of neutral and acidic 5-methoxyindoles and some related compounds,
Biomed. Mass Spectrom., 1980, 7, 7, 294-300, https://doi.org/10.1002/bms.1200070706
. [all data]
Notes
Go To: Top, Gas Chromatography, References
- Data from NIST Standard Reference Database 69: NIST Chemistry WebBook
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