Androstane-3,17-diol, (3α,5β,17β)-, 2TMS derivative


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
PackedOV-101207.2558.Berthou, Picart, et al., 1976Column length: 2.1 m
CapillaryOV-101252.2572.Berthou, Picart, et al., 1976Column length: 55. m; Column diameter: 0.37 mm
CapillaryOV-101252.2572.Berthou, Picart, et al., 1976Column length: 55. m; Column diameter: 0.37 mm
PackedOV-1210.2550.Brooks, Thawley, et al., 1971 

Van Den Dool and Kratz RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryBP-12570.Houghton, Ginn, et al., 1989H2, 5. K/min; Column length: 25. m; Column diameter: 0.3 mm; Tstart: 150. C; Tend: 300. C
CapillaryBP-52581.Houghton, Ginn, et al., 1989H2, 5. K/min; Column length: 25. m; Column diameter: 0.3 mm; Tstart: 150. C; Tend: 300. C

Normal alkane RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
PackedSE-302545.Harvey, 1977N2, Gas Chrom Q, 2. K/min; Column length: 2.0 m; Tstart: 150. C; Tend: 290. C

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryDB-12567.Bowers, 199630. m/0.25 mm/0.25 μm, Helium; Program: 170 0C 20 0C/min -> 260 0C 2.7 0C/min -> 305 0C 20 0C/min -> 320 0C

References

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Berthou, Picart, et al., 1976
Berthou, F.; Picart, D.; Bardou, L.; Floch, H.H., Separation of C19 and C21 dihydroxysteroids by open-hole tubular glass columns and lipophilic gel chromatography, J. Chromatogr., 1976, 118, 2, 135-155, https://doi.org/10.1016/S0021-9673(00)81203-0 . [all data]

Brooks, Thawley, et al., 1971
Brooks, C.J.W.; Thawley, A.R.; Rocher, P.; Middleditch, B.S.; Anthony, G.M.; Stillwell, W.G., Characterization of Steroidal Drug Metabolites by Combined Gas Chromatography-Mass Spectrometry, J. Chromatogr. Sci., 1971, 9, 1, 35-43, https://doi.org/10.1093/chromsci/9.1.35 . [all data]

Houghton, Ginn, et al., 1989
Houghton, E.; Ginn, A.; Teale, P.; Dumasia, M.C.; Copsey, J., Comparison of the use of mass spectrometry and methylene unit values in the determination of the stereochemistry of estranediol, the major urinary metabolite of 19-nortestosterone in the horse, J. Chromatogr., 1989, 479, 73-83, https://doi.org/10.1016/S0021-9673(01)83318-5 . [all data]

Harvey, 1977
Harvey, D.J., Allyldimethylsilyl ethers as derivatives for the characterization of steroids and cannabinoids by combined gas chromatography and mass spectrometry, Biomed. Mass Spectrom., 1977, 4, 4, 265-274, https://doi.org/10.1002/bms.1200040414 . [all data]

Bowers, 1996
Bowers, L.D., Separation and confirmation of anabolic steroids with quadrupole ion trap tandem mass spectrometry, J. Chromatogr. B, 1996, 687, 1, 69-78, https://doi.org/10.1016/S0378-4347(96)00231-9 . [all data]


Notes

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