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4-Cholesten-7-«alpha»-ol-3-one, TMS


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryMethyl Silicone3235.Shoda, Axelson, et al., 199325. m/0.32 mm/0.25 «mu»m, 30. K/min; Tstart: 60. C; Tend: 290. C

Kovats' RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillarySE-303230.Axelson, Mörk, et al., 199130. m/0.32 mm/0.25 «mu»m; Program: 50C(6min) => rapidly => 190C => 5C/min => 285C

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryUltra-13200.Axelson, Ellis, et al., 200025. m/0.32 mm/0.17 «mu»m, H2; Program: 50 0C (3 min) -> 185 0C 5 K/min -> 280 0C
CapillaryUltra-13210.Axelson and Larsson, 199625. m/0.32 mm/0.17 «mu»m; Program: 50C (3min) => (rapidly) => 185C => 5C/min => 280C
CapillaryOV-13210.Brooks, Cole, et al., 198325. m/0.25 mm/0.25 «mu»m; Program: 80 0C (5 min) 40 K/min -> 120 0C (2 min) 2 K/min -> 200 0C (for androstanes), or 80 0C (5 min) 40 K/min -> 190 0C (2 min) 2 K/min -> 270 0C (for sterols)

References

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Shoda, Axelson, et al., 1993
Shoda, J.; Axelson, M.; Sjövall, J., Synthesis of potential C27-intermediates in bile acid biosynthesis and their deuterium-labeled analogs, Steroids, 1993, 58, 3, 119-125, https://doi.org/10.1016/0039-128X(93)90048-R . [all data]

Axelson, Mörk, et al., 1991
Axelson, M.; Mörk, B.; Everson, G.T., Bile Acid Synthesis in Cultured Human Hepatoblastoma Cells, J. Biol. Chem., 1991, 266, 27, 17770-17777. [all data]

Axelson, Ellis, et al., 2000
Axelson, M.; Ellis, E.; Mörk, B.; Garmark, K.; Abrahamsson, A.; Björkhem, I.; Ericzon, B.-G.; Einarsson, C., Bile acid synthesis in cultured human hepatocytes: support for an alternative biosynthetic pathway to cholic acid, Hepatology, 2000, 31, 6, 1305-1312, https://doi.org/10.1053/jhep.2000.7877 . [all data]

Axelson and Larsson, 1996
Axelson, M.; Larsson, O., 27-Hydroxylated low density lipoprotein (LDL) cholesterol can be converted to 7«alpha»,27-Dihydroxy-4-cholesten-3-one (cytosterone) before suppressing cholesterol production in normal human fibroblasts. Evidence that an altered metabolism of LDL cholesterol can underlie a defective feedback control in malignant cells, J. Biol. Chem., 1996, 271, 22, 12724-12736. [all data]

Brooks, Cole, et al., 1983
Brooks, C.J.W.; Cole, W.J.; Lawrie, T.D.V.; MacLachlan, J.; Borthwick, J.H.; Barrett, G.M., Selective reactions in the analytical characterisation of steroids by gas chromatography-mass spectrometry, J. Steroid Biochem., 1983, 19, 1, 189-201, https://doi.org/10.1016/S0022-4731(83)80025-9 . [all data]


Notes

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