5α-Androstan-3α-ol-17-one, TMS derivative


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
PackedOV-1230.2485.Quilliam and Westmore, 1978Gas Chrom Q; Column length: 1. m
PackedOV-1225.2458.Voelter, Jung, et al., 1971N2, GasChrom Q

Van Den Dool and Kratz RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryDB-12505.Ludwig-Koehn and Henning, 198630. m/0.25 mm/0.25 μm, H2, 2. K/min; Tstart: 120. C; Tend: 280. C

Normal alkane RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
CapillaryOV-101240.2499.Krupcik, Rutten, et al., 1976Column length: 30. m; Column diameter: 0.25 mm
CapillaryOV-101251.2515.Krupcik, Rutten, et al., 1976Column length: 30. m; Column diameter: 0.25 mm

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryMethyl Silicone2452.Meng and Sjövall, 199725. m/0.32 mm/0.25 μm; Program: 60 0C 30 K/min -> 210 0C (1.5 min) 2.5 K/min -> 320 0C (20 min)

References

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Quilliam and Westmore, 1978
Quilliam, M.A.; Westmore, J.B., Sterically crowded trialkylsilyl derivatives for chromatography and mass spectrometry of biologically-important compounds, Anal. Chem., 1978, 50, 1, 59-68, https://doi.org/10.1021/ac50023a020 . [all data]

Voelter, Jung, et al., 1971
Voelter, W.; Jung, G.; Breitmaier, E.; Bouchon, G.; Bayer, E.; Gupta, D., Comparison of the trifluoroacetyl and trimethylsilylether derivaties for gas chromatography of steroids, Anal. Chim. Acta., 1971, 53, 1, 185-188, https://doi.org/10.1016/S0003-2670(01)80085-4 . [all data]

Ludwig-Koehn and Henning, 1986
Ludwig-Koehn, H.; Henning, H.V., Some aspects of quantification of neutral steroid metabolites in body fluids of healthy and uremic subjects by capillary gas chromatography, J. Hi. Res. Chromatogr. Chromatogr. Comm., 1986, 9, 1, 35-38, https://doi.org/10.1002/jhrc.1240090106 . [all data]

Krupcik, Rutten, et al., 1976
Krupcik, J.; Rutten, G.A.F.M.; Rijks, J.A., The influence of temperature on the separation and characterization of steroids on non-polar glass capillary columns, Chem. zvesti, 1976, 30, 4, 469-479. [all data]

Meng and Sjövall, 1997
Meng, L.-J.; Sjövall, J., Method for combined analysis of profiles of conjugated progesterone metabolites and bile acids in serum and urine of pregnant women, J. Chromatogr. B, 1997, 688, 1, 11-26, https://doi.org/10.1016/S0378-4347(97)88051-6 . [all data]


Notes

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