Homogentisic acid, 3TMS derivative


Van Den Dool and Kratz RI, non-polar column, temperature ramp

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase DB-5DB-5DB-5PE-5htPE-5ht
Column length (m) 30.30.30.30.30.
Carrier gas HeHeHeHeHe
Substrate      
Column diameter (mm) 0.250.250.250.250.25
Phase thickness (μm) 0.250.250.250.10.1
Tstart (C) 50.50.50.50.50.
Tend (C) 320.320.320.320.320.
Heat rate (K/min) 3.3.3.3.3.
Initial hold (min)      
Final hold (min)      
I 1860.1863.1864.1863.1867.
ReferenceJaroszynska and Ligor, 2005Jaroszynska and Ligor, 2005Jaroszynska and Ligor, 2005Jaroszynska and Ligor, 2005Jaroszynska and Ligor, 2005
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase PE-5htPE-5htPE-5msDB-5DB-5
Column length (m) 30.30.30.30.30.
Carrier gas HeHeHeHeHe
Substrate      
Column diameter (mm) 0.250.250.250.250.25
Phase thickness (μm) 0.10.10.250.250.25
Tstart (C) 50.50.50.50.50.
Tend (C) 320.320.320.240.240.
Heat rate (K/min) 3.3.3.3.3.
Initial hold (min)      
Final hold (min)      
I 1867.1867.1839.1855.1864.
ReferenceJaroszynska and Ligor, 2005Jaroszynska and Ligor, 2005Jaroszynska and Ligor, 2005Jaroszynska, 2003Jaroszynska, 2003
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase DB-5DB-5CP Sil 5 CBDB-5OV-1
Column length (m) 30.30.25.30.10.
Carrier gas HeHe HeHe
Substrate      
Column diameter (mm) 0.250.250.150.250.22
Phase thickness (μm) 0.250.250.121.0.17
Tstart (C) 50.50.90.100.70.
Tend (C) 240.240.270.290.270.
Heat rate (K/min) 7.7.10.4.5.
Initial hold (min)   1.4. 
Final hold (min)   7.10. 
I 1852.1861.1850.1850.1860.
ReferenceJaroszynska, 2003Jaroszynska, 2003Halket, Przyborowska, et al., 1999Kimura, Yamamoto, et al., 1999Lefevere, Verhaeghe, et al., 1989
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryPacked
Active phase SE-52OV-1
Column length (m) 25.2.
Carrier gas HeHe
Substrate  Chromosorb W HP (80-100 mesh)
Column diameter (mm) 0.32 
Phase thickness (μm) 0.52 
Tstart (C) 70.100.
Tend (C) 270.325.
Heat rate (K/min) 5.10.
Initial hold (min)   
Final hold (min)   
I 1859.1841.
ReferenceLefevere, Verhaeghe, et al., 1989Butts, 1972
Comment MSDC-RI MSDC-RI

References

Go To: Top, Van Den Dool and Kratz RI, non-polar column, temperature ramp, Notes

Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Jaroszynska and Ligor, 2005
Jaroszynska, J.; Ligor, T., The identification of phenolic compounds by a gas chromatographic method on three capillary columns with the same non-polar stationary phase, Anal. Chim. Acta., 2005, 539, 1-2, 11-15, https://doi.org/10.1016/j.aca.2005.03.024 . [all data]

Jaroszynska, 2003
Jaroszynska, J., Isolation of free phenolic compounds from arboreal leaves by use of the Florisil/C18 system, Anal. Bioanal. Chem., 2003, 377, 4, 702-708, https://doi.org/10.1007/s00216-003-2155-z . [all data]

Halket, Przyborowska, et al., 1999
Halket, J.N.; Przyborowska, A.; Stein, S.E.; Mallard, W.G.; Down, S.; Chalmers, R., Deconvolution gas chromatography / mass spectrometry of urinary organic acids - potential for pattern recognition and automated identification of metabolic disorders, Rapid Commun. Mass spectrom., 1999, 13, 4, 279-284, https://doi.org/10.1002/(SICI)1097-0231(19990228)13:4<279::AID-RCM478>3.0.CO;2-I . [all data]

Kimura, Yamamoto, et al., 1999
Kimura, M.; Yamamoto, T.; Yamaguchi, S., Automated metabolic profiling and interpretation of GC/MS data for organic acidemia screening: a personal computer-based system, Tohoku J. Exp. Med., 1999, 188, 4, 317-334, https://doi.org/10.1620/tjem.188.317 . [all data]

Lefevere, Verhaeghe, et al., 1989
Lefevere, M.F.; Verhaeghe, B.J.; Declerck, D.H.; Van Bocxlaer, J.F.; De Leenheer, A.P.; De Sagher, R.M., Metabolic Profiling of Urinary Organic Acids by Single and Multicolumn Capillary Gas Chromatography, J. Chromatogr. Sci., 1989, 27, 1, 23-29, https://doi.org/10.1093/chromsci/27.1.23 . [all data]

Butts, 1972
Butts, W.C., Two-Column Gas Chromatography of Trimethylsilyl Derivatives of Biochemically Significant Compounds, Anal. Biochemistry, 1972, 46, 1, 187-199, https://doi.org/10.1016/0003-2697(72)90411-3 . [all data]


Notes

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