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Cholesterol, TMS derivative


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
PackedOV-1260.3136.Quilliam and Westmore, 1978Gas Chrom Q; Column length: 1. m
CapillaryOV-1255.3124.Luyten and Rutten, 1974He; Column length: 20. m; Column diameter: 0.25 mm

Kovats' RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryMethyl Silicone3143.Shoda, Axelson, et al., 199325. m/0.32 mm/0.25 «mu»m, 30. K/min; Tstart: 60. C; Tend: 290. C

Kovats' RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillarySE-303135.Axelson, Mörk, et al., 199130. m/0.32 mm/0.25 «mu»m; Program: 50C(6min) => rapidly => 190C => 5C/min => 285C

Van Den Dool and Kratz RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryDB-5MS3144.9Jonsson, Johansson, et al., 200510. m/0.18 mm/0.18 «mu»m, 70. C @ 2. min, 40. K/min, 320. C @ 2. min
CapillaryOV-13120.Luyten and Rutten, 1974He, 1. K/min; Column length: 20. m; Column diameter: 0.25 mm; Tstart: 220. C
CapillarySE-303122.Luyten and Rutten, 1974He, 1. K/min; Column length: 20. m; Column diameter: 0.25 mm; Tstart: 200. C
PackedSE-303101.Luyten and Rutten, 1974He, 1. K/min; Column length: 20. m; Column diameter: 0.25 mm; Tstart: 180. C

Normal alkane RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryRTX-5 Sil MS3155.Lytovchenko, Beleggia, et al., 200930. m/0.25 mm/0.25 «mu»m, Helium, 70. C @ 5. min, 5. K/min, 310. C @ 1. min
CapillaryMethyl Silicone3132.Ichimiya, Egestad, et al., 199125. m/0.32 mm/0.25 «mu»m, He, 25. K/min; Tstart: 80. C; Tend: 280. C

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryUltra-13110.Axelson, Ellis, et al., 200025. m/0.32 mm/0.17 «mu»m, H2; Program: 50 0C (3 min) -> 185 0C 5 K/min -> 280 0C

References

Go To: Top, Gas Chromatography, Notes

Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Quilliam and Westmore, 1978
Quilliam, M.A.; Westmore, J.B., Sterically crowded trialkylsilyl derivatives for chromatography and mass spectrometry of biologically-important compounds, Anal. Chem., 1978, 50, 1, 59-68, https://doi.org/10.1021/ac50023a020 . [all data]

Luyten and Rutten, 1974
Luyten, J.A.; Rutten, G.A.F.M., Analysis of Steroids by High-Resolution Gas-Liquid Chromatography. II. Application to Urinary Samples, J. Chromatogr., 1974, 91, 393-406, https://doi.org/10.1016/S0021-9673(01)97918-X . [all data]

Shoda, Axelson, et al., 1993
Shoda, J.; Axelson, M.; Sjövall, J., Synthesis of potential C27-intermediates in bile acid biosynthesis and their deuterium-labeled analogs, Steroids, 1993, 58, 3, 119-125, https://doi.org/10.1016/0039-128X(93)90048-R . [all data]

Axelson, Mörk, et al., 1991
Axelson, M.; Mörk, B.; Everson, G.T., Bile Acid Synthesis in Cultured Human Hepatoblastoma Cells, J. Biol. Chem., 1991, 266, 27, 17770-17777. [all data]

Jonsson, Johansson, et al., 2005
Jonsson, P.; Johansson, A.I.; Gullberg, J.; Trygg, J.; Jiye, A.; Grung, B.; Marklund, S.; Sjöström, M.; Antti, H.; Moritz, T., High-throughput data analysis for detecting and identifying differences between samples in GC/MS-based metabolomic analyses, Anal. Chem., 2005, 77, 17, 5635-5642, https://doi.org/10.1021/ac050601e . [all data]

Lytovchenko, Beleggia, et al., 2009
Lytovchenko, A.; Beleggia, R.; Schauer, N.; Isaacson, T.; Leuendorf, J.E.; Hellmann, H.; Rose, J.K.C.; Fernie, A.R., Application of GC-MS fpr the detection of lipophilic compounds in diverse plant tissues, Plant Methods, 2009, 5, 4, 1-11. [all data]

Ichimiya, Egestad, et al., 1991
Ichimiya, H.; Egestad, B.; Nazer, H.; Baginski, E.S.; Clayton, P.T.; Sjövall, J., Bile acids and bile alcohols in a child with hepatic 3«beta»-hydroxy-«DELTA»5-C27-steroid dehydrogenase deficiency: effects of chenodeoxycholic acid treatment, J. Lipid Res., 1991, 32, 829-841. [all data]

Axelson, Ellis, et al., 2000
Axelson, M.; Ellis, E.; Mörk, B.; Garmark, K.; Abrahamsson, A.; Björkhem, I.; Ericzon, B.-G.; Einarsson, C., Bile acid synthesis in cultured human hepatocytes: support for an alternative biosynthetic pathway to cholic acid, Hepatology, 2000, 31, 6, 1305-1312, https://doi.org/10.1053/jhep.2000.7877 . [all data]


Notes

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