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GA20, Me-TMS


Van Den Dool and Kratz RI, non-polar column, custom temperature program

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase HP-1HP-1HP-1HP-1DB-5
Column length (m) 30.30.12.12.15.
Carrier gas HeHe   
Substrate      
Column diameter (mm) 0.250.250.220.220.25
Phase thickness (mum) 0.250.25   
Program 60C (1min) => 15C/min => 200C => 5C/min => 285C60C (1min) => 15C/min => 200C => 5C/min => 285C60C(0.5min) => 20C/min => 200C => 5C/min => 300C60C(0.5min) => 20C/min => 200C => 5C/min => 300C60C(0.5min) => 20C/min => 200C => 5C/min => 300C
I 2485.2485.2494.2496.2508.
ReferenceKim, Lee, et al., 2003Kim, Lee, et al., 2003Pearce, Hutt, et al., 2002Pearce, Hutt, et al., 2002Pearce, Hutt, et al., 2002
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase DB-5DB-5MSDB-5MSOV-1CP Sil 5 CB
Column length (m) 15.30.30.25.25.
Carrier gas  HeHeHeHe
Substrate      
Column diameter (mm) 0.250.250.250.20.25
Phase thickness (mum)  0.250.25  
Program 60C(0.5min) => 20C/min => 200C => 5C/min => 300C50C(1min) => 30C/min => 240C => 10C/min => 280C50C(1min) => 30C/min => 240C => 10C/min => 280C60C(2min) => 13C/min => 150C => 3C/min => 280C(10min)60C(0.7min) => 20C/min => 220C => 4C/min => 300C
I 2510.2549.2549.2488.2548.
ReferencePearce, Hutt, et al., 2002Gutiérrez-Mañero, Ramos-Solano, et al., 2001Gutiérrez-Mañero, Ramos-Solano, et al., 2001Kobayashi, MacMillan, et al., 2000Taylor, Blake, et al., 2000
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase CP Sil 5 CBOV-1CP-Sil5 CB MSCP-Sil5 CB MSOV-1
Column length (m) 25.25.25.25.25.
Carrier gas HeHeHeHeHe
Substrate      
Column diameter (mm) 0.250.20.250.250.2
Phase thickness (mum)  0.25  0.25
Program 60C(0.7min) => 20C/min => 220C => 4C/min => 300C60C(2min) => 10C/min => 150C => 3C/min => 300C(10min)90C(1min) => 20C/min => 220C => 4C/min => 290C90C(1min) => 20C/min => 220C => 4C/min => 290C60C(2min) => 10min/C => 150C => 3C/min => 300C
I 2548.2482.2517.2517.2482.
ReferenceTaylor, Blake, et al., 2000Davis, Kobayashi, et al., 1999Roberts, Blake, et al., 1999Roberts, Blake, et al., 1999Davis, Kobayashi, et al., 1998
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase DB-1OV-1BP-1BP-1DB-1
Column length (m) 15.25.25.25.30.
Carrier gas     He
Substrate      
Column diameter (mm) 0.250.20.20.20.25
Phase thickness (mum) 0.330.250.10.1 
Program 120(2min) => (16C/min) => 216(5min) => (8C/min) => 280C60C(2min) => 10C/min => 150C => 3C/min => 300C60C(2min) => 10C/min => 150C => 3C/min => 300C60C(2min) => 10C/min => 150C => 3C/min => 300C40C (2min) => 10C/min => 150C => 3C/min => 300C
I 2485.2482.2493.2494.2485.
ReferenceHisamatsu, Koshioka, et al., 1998Webb, Appleford, et al., 1998Webb, Appleford, et al., 1998Webb, Appleford, et al., 1998Grünzweig, Rabinowitch, et al., 1997
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase DB-1CP Sil 5 CBCP Sil 5 CBDB-1OV-1
Column length (m) 30.30.30.15.25.
Carrier gas HeHeHeHe 
Substrate      
Column diameter (mm) 0.250.250.250.250.2
Phase thickness (mum)  0.40.40.250.25
Program 40C (2min) => 10C/min => 150C => 3C/min => 300C200C => 25C/min => 250C => 4C/min => 280C(4min)200C => 25C/min => 250C => 4C/min => 280C(4min)80C(1min) => 30C/min => 245C => 5C/min => 300C50C(2min) => 10C/min => 150C => 3C/min => 300C
I 2487.2501.2501.2490.2491.
ReferenceGrünzweig, Rabinowitch, et al., 1997Kappers, Jordi, et al., 1997Kappers, Jordi, et al., 1997Rijnders, Yang, et al., 1997Kobayashi, Spray, et al., 1996
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase OV-1DB-1DB-1OV-1OV-1
Column length (m) 25.15.15.25.25.
Carrier gas  HeHe  
Substrate      
Column diameter (mm) 0.20.250.250.20.2
Phase thickness (mum) 0.250.250.250.250.25
Program 50C(2min) => 10C/min => 150C => 3C/min => 300C60C(1min) => 20C/min => 200C => 4C/min => 260C60C(1min) => 20C/min => 200C => 4C/min => 260C60C(2min) => 10C/min => 150C => 3C/min => 300C60C(2min) => 10C/min => 150C => 3C/min => 300C
I 2492.2488.2488.2482.2492.
ReferenceKobayashi, Spray, et al., 1996Ridoutt, Pharis, et al., 1995Ridoutt, Pharis, et al., 1995Santes, Hedden, et al., 1995Santes, Hedden, et al., 1995
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase OV-1OV-1CP Sil 5 CBCP Sil 5 CBBP-1
Column length (m) 25.25.25.25.25.
Carrier gas HeHeHeHeHe
Substrate      
Column diameter (mm) 0.20.20.250.250.22
Phase thickness (mum)     0.25
Program 60C(2min) => 13C/min => 150C => 3C/min => 280C(10min)60C(2min) => 13C/min => 150C => 3C/min => 280C(10min)50C(1min) => 15C/min => 220C => 2C/min => 300C50C(1min) => 15C/min => 220C => 2C/min => 300C60C => 30C/min => 240C => 3C/min => 290C
I 2495.2496.2517.2517.2520.
ReferenceTalo, Spray, et al., 1995Talo, Spray, et al., 1995Blake and Browning, 1994Blake and Browning, 1994Hasan, Ridoutt, et al., 1994
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase BP-1OV-1DB-1CP Sil 5 CBCP Sil 5 CB
Column length (m) 25.25.15.25.25.
Carrier gas HeHe HeHe
Substrate      
Column diameter (mm) 0.220.20.220.250.25
Phase thickness (mum) 0.25 0.25  
Program 60C => 30C/min => 240C => 3C/min => 290C60C(2min) => 13C/min => 150C => 3C/min => 280C(10min)60(0.1 min)-200 0C at 20 deg/min; 200-300 0C at 5 deg/min60C(0.3min) => 20C/min => 220C => 4C/min => 300C60C(0.3min) => 20C/min => 220C => 4C/min => 300C
I 2521.2490.2499.2516.2517.
ReferenceHasan, Ridoutt, et al., 1994Kobayashi, Gaskin, et al., 1994Pearce, Koshioka, et al., 1994Taylor, Blake, et al., 1994Taylor, Blake, et al., 1994
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase SE-52SE-52OV-1SPB-1SPB-1
Column length (m) 25.25.25.30.30.
Carrier gas HeHe HeHe
Substrate      
Column diameter (mm) 0.220.220.220.250.25
Phase thickness (mum) 0.250.250.250.250.25
Program 50C => 15C/min => 240C => 4C/min => 300C50C => 15C/min => 240C => 4C/min => 300C60C(2min) => 10C/min => 150C => 3C/min => 310C60C(2min) => 20C/min => 160C => 5C/min => 290C60C(2min) => 20C/min => 160C => 5C/min => 290C
I 2477.2478.2483.2502.2502.
ReferenceHedden, Hoad, et al., 1993Hedden, Hoad, et al., 1993Waycott, Smith, et al., 1991Poling and Maier, 1988Poling and Maier, 1988
Comment MSDC-RI MSDC-RI MSDC-RI MSDC-RI MSDC-RI
Column type CapillaryCapillary
Active phase SPB-1OV-1
Column length (m) 30.25.
Carrier gas HeHe
Substrate   
Column diameter (mm) 0.250.2
Phase thickness (mum) 0.25 
Program 60C(2min) => 20C/min => 160C => 5C/min => 290C60C(2min) => 13C/min => 150C => 3C/min => 280C(10min)
I 2502.2474.
ReferencePoling and Maier, 1988Gaskin, Gilmour, et al., 1984
Comment MSDC-RI MSDC-RI

References

Go To: Top, Van Den Dool and Kratz RI, non-polar column, custom temperature program, Notes

Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Kim, Lee, et al., 2003
Kim, S.-K.; Lee, S.-C.; Shin, D.-H.; Jang, S.-W.; Nam, J.-W.; Park, T.-S.; Lee, I.-J., Quantification of endogenous gibberellins in leaves and tubers of Chinese yam, Dioscorea opposita Thunb. cv. Tsukune during tuber enlargement, Plant Growth Regul., 2003, 39, 2, 125-130, https://doi.org/10.1023/A:1022569506883 . [all data]

Pearce, Hutt, et al., 2002
Pearce, D.W.; Hutt, O.E.; Rood, S.B.; Mander, L.N., Gibberellins in shoots and developing capsules of Populus species, Phytochemistry, 2002, 59, 6, 679-687, https://doi.org/10.1016/S0031-9422(02)00009-2 . [all data]

Gutiérrez-Mañero, Ramos-Solano, et al., 2001
Gutiérrez-Mañero, F.J.; Ramos-Solano, B.; Probanza, A.; Mehouachi, J.; Tadeo, R.; Talon, M., The plant-growth-promoting rhizobacteria Bacillus pumilus and Baillus licheniformis produce high amounts of physiologically active gibberellins, Physiol. Plant., 2001, 111, 2, 206-211, https://doi.org/10.1034/j.1399-3054.2001.1110211.x . [all data]

Kobayashi, MacMillan, et al., 2000
Kobayashi, M.; MacMillan, J.; Phinney, B.; Gaskin, P.; Spray, C.R.; Hedden, P., Gibberellin biosynthesis: metabolic evidence for three steps in the early 13-hydroxylation pathway of rice, Phytochemistry, 2000, 55, 4, 317-321, https://doi.org/10.1016/S0031-9422(00)00265-X . [all data]

Taylor, Blake, et al., 2000
Taylor, D.R.; Blake, P.S.; Crisp, C.M., Identification of gibberellins in leaf tissues of day-neutral strawberry (Fragaria × ananassa Duch.) cultivars, Plant Growth Regul., 2000, 30, 1, 5-7, https://doi.org/10.1023/A:1006275024517 . [all data]

Davis, Kobayashi, et al., 1999
Davis, G.; Kobayashi, M.; Phinney, B.O.; Lange, T.; Croker, S.J.; Gaskin, P.; MacMillan, J., Gibberellin biosynthesis in maize. Metabolic studies with GA15, GA24, GA25, GA7, and 2,3-dehydro-GA9, Plant Physiol., 1999, 121, 3, 1037-1045, https://doi.org/10.1104/pp.121.3.1037 . [all data]

Roberts, Blake, et al., 1999
Roberts, A.V.; Blake, P.S.; Lewis, R.; Taylor, J.M.; Dunstan, D.I., The effect of gibberellins on flowering in roses, J. Plant Growth Regul., 1999, 18, 3, 113-119, https://doi.org/10.1007/PL00007058 . [all data]

Davis, Kobayashi, et al., 1998
Davis, G.; Kobayashi, M.; Phinney, B.O.; MacMillan, J.; Gaskin, P., The Metabolism of GA9 in maize (ZEA mays), Phytochemistry, 1998, 47, 4, 635-639, https://doi.org/10.1016/S0031-9422(97)87974-5 . [all data]

Hisamatsu, Koshioka, et al., 1998
Hisamatsu, T.; Koshioka, M.; Kubota, S.; Nishijima, T.; Yamane, H.; King, R.W.; Mander, L.N., Isolation and identification of GA112 (12«beta»-hydroxy-GA12) in Matthiola incana, Phytochemistry, 1998, 47, 1, 3-6, https://doi.org/10.1016/S0031-9422(97)00543-8 . [all data]

Webb, Appleford, et al., 1998
Webb, S.E.; Appleford, N.E.J.; Gaskin, P.; Lenton, J.R., Gibberellins in internodes and ears of wheat containing different dwarfing alleles, Phytochemistry, 1998, 47, 5, 671-677, https://doi.org/10.1016/S0031-9422(97)00684-5 . [all data]

Grünzweig, Rabinowitch, et al., 1997
Grünzweig, J.M.; Rabinowitch, H.D.; Katan, J.; Wodner, M.; Ben-Tal, Y., Endogenous gibberellins in foliage of tomato (Lycopersicon esculentum), Phytochemistry, 1997, 46, 5, 811-815, https://doi.org/10.1016/S0031-9422(97)00383-X . [all data]

Kappers, Jordi, et al., 1997
Kappers, I.F.; Jordi, W.; Maas, F.M.; van der Plas, L.H.W., Gibberellins in leaves of Alstroemeria hybrida: identification and quantification in relation to leaf age, J. Plant Growth Regul., 1997, 16, 4, 219-225, https://doi.org/10.1007/PL00007000 . [all data]

Rijnders, Yang, et al., 1997
Rijnders, J.G.H.M.; Yang, Y.-Y.; Kamiya, Y.; Takahashi, N.; Barendse, G.W.M.; Blom, C.W.P.M.; Voesenek, L.A.C.J., Ethylene enhances gibberellin levels and petiole sensitivity in flooding-tolerant Rumex palustris but not in flooding-intolerant R. acetosa, Planta, 1997, 203, 1, 20-25, https://doi.org/10.1007/s00050160 . [all data]

Kobayashi, Spray, et al., 1996
Kobayashi, M.; Spray, C.R.; Phinney, B.O.; Gaskin, P.; MacMillan, J., Gibberellin metabolism in maize. The stepwise conversion of gibberellin A12-aldehyde to gibberellin A20, Plant Physiol., 1996, 110, 413-418. [all data]

Ridoutt, Pharis, et al., 1995
Ridoutt, B.G.; Pharis, R.P.; Sands, R., Identification and quantification of cambial region hormones of Eucalyptus globulus, Plant Cell Physiol., 1995, 36, 6, 1143-1147. [all data]

Santes, Hedden, et al., 1995
Santes, C.M.; Hedden, P.; Gaskin, P.; Garcia-Martinez, J.L., Gibberellins and related compounds in young fruits of pea and their relationship to fruit-set, Phytochemistry, 1995, 40, 5, 1347-1355, https://doi.org/10.1016/0031-9422(95)00489-T . [all data]

Talo, Spray, et al., 1995
Talo, A.; Spray, C.R.; Somers, D.A.; Donovan, C.M.; Gaskin, P.; Macmillian, J.; Phinney, B.O., Endogenous gibberellins from callus cultures of maize, Phytochemistry, 1995, 40, 1, 11-15, https://doi.org/10.1016/0031-9422(95)00000-W . [all data]

Blake and Browning, 1994
Blake, P.S.; Browning, G., Gibberellins in developing fruits of Prunus avium, Phytochemistry, 1994, 35, 6, 1383-1386, https://doi.org/10.1016/S0031-9422(00)86860-0 . [all data]

Hasan, Ridoutt, et al., 1994
Hasan, O.; Ridoutt, B.G.; Ross, J.J.; Davies, N.W.; Reid, J.B., Identification and quantification of endogenous gibberellins in apical buds and the cambial region of Eucalyptus, Physiol. Plant., 1994, 90, 3, 475-480, https://doi.org/10.1111/j.1399-3054.1994.tb08804.x . [all data]

Kobayashi, Gaskin, et al., 1994
Kobayashi, M.; Gaskin, P.; Spray, C.R.; Phinney, B.O.; MacMillan, J., The metabolism of gibberellin A20 to gibberellin A1 by tall and dwarf mutants of Oryza sativa and Arabidopsis thaliana, Plant Physiol., 1994, 106, 1367-1372. [all data]

Pearce, Koshioka, et al., 1994
Pearce, D.W.; Koshioka, M.; Pharis, R.P., Chromatography of gibberellins, J. Chromatogr. A, 1994, 658, 1, 91-122, https://doi.org/10.1016/0021-9673(94)85210-3 . [all data]

Taylor, Blake, et al., 1994
Taylor, D.R.; Blake, P.S.; Browning, G., Identification of gibberellins in leaf tissues of strawberry (Fragaria × ananassa Duch.) grown under different photoperiods, Plant Growth Regul., 1994, 15, 3, 235-240, https://doi.org/10.1007/BF00029896 . [all data]

Hedden, Hoad, et al., 1993
Hedden, P.; Hoad, G.V.; Gaskin, P.; Lewis, M.J.; Green, J.R.; Furber, M.; Mander, L.N., Kaurenoids and gibberellins, including the newly characterized gibberellin A88, in developing apple seeds, Phytochemistry, 1993, 32, 2, 231-237, https://doi.org/10.1016/S0031-9422(00)94973-2 . [all data]

Waycott, Smith, et al., 1991
Waycott, W.; Smith, V.A.; Gaskin, P.; MacMillan, J.; Taiz, L., The endogenous gibberellins of dwarf mutants of lettuce, Plant Physiol., 1991, 95, 4, 1169-1173, https://doi.org/10.1104/pp.95.4.1169 . [all data]

Poling and Maier, 1988
Poling, S.M.; Maier, V.P., Identification of endogenous gibberellins in navel orange shoots, Plant Physiol., 1988, 88, 3, 639-642, https://doi.org/10.1104/pp.88.3.639 . [all data]

Gaskin, Gilmour, et al., 1984
Gaskin, P.; Gilmour, S.J.; Lenton, J.R.; MacMillan, J.; Sponsel, V.M., Endogenous gibberellins and kauranoids identified from developing and germinating barley grain, J. Plant Growth Regul., 1984, 2, 1-4, 229-242, https://doi.org/10.1007/BF02042252 . [all data]


Notes

Go To: Top, Van Den Dool and Kratz RI, non-polar column, custom temperature program, References