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5-«alpha»-Pregnan-3-«beta»,20-«alpha»-diol, TMS


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
PackedOV-101207.2823.Berthou, Picart, et al., 1976Column length: 2.1 m
CapillaryOV-101252.2848.Berthou, Picart, et al., 1976Column length: 55. m; Column diameter: 0.37 mm

Normal alkane RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
PackedSE-302847.Harvey, 1980N2, Gas Chrom Q, 4. K/min; Column length: 2. m; Tstart: 150. C; Tend: 300. C
PackedSE-302847.Harvey, 1977N2, Gas Chrom Q, 2. K/min; Column length: 2.0 m; Tstart: 150. C; Tend: 290. C

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryMethyl Silicone2836.Meng and Sjövall, 199725. m/0.32 mm/0.25 «mu»m; Program: 60 0C 30 K/min -> 210 0C (1.5 min) 2.5 K/min -> 320 0C (20 min)
CapillaryCross-Linked Methylsilicone2854.Meng, Griffiths, et al., 199625. m/0.32 mm/0.32 «mu»m; Program: 60C => 30C/min => 210C (1.5min) => 2.5C/min => 320C (20min)
CapillaryPolydimethyl siloxane2886.Vandenheuvel, 1975Program: not specified

References

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Berthou, Picart, et al., 1976
Berthou, F.; Picart, D.; Bardou, L.; Floch, H.H., Separation of C19 and C21 dihydroxysteroids by open-hole tubular glass columns and lipophilic gel chromatography, J. Chromatogr., 1976, 118, 2, 135-155, https://doi.org/10.1016/S0021-9673(00)81203-0 . [all data]

Harvey, 1980
Harvey, D.J., Vinyldimethylsilyl ethers as derivative for the characterization of steroids and cannabinoids by gas chromatography mass spectrometry, Biomed. Mass Spectrom., 1980, 7, 5, 211-216, https://doi.org/10.1002/bms.1200070507 . [all data]

Harvey, 1977
Harvey, D.J., Allyldimethylsilyl ethers as derivatives for the characterization of steroids and cannabinoids by combined gas chromatography and mass spectrometry, Biomed. Mass Spectrom., 1977, 4, 4, 265-274, https://doi.org/10.1002/bms.1200040414 . [all data]

Meng and Sjövall, 1997
Meng, L.-J.; Sjövall, J., Method for combined analysis of profiles of conjugated progesterone metabolites and bile acids in serum and urine of pregnant women, J. Chromatogr. B, 1997, 688, 1, 11-26, https://doi.org/10.1016/S0378-4347(97)88051-6 . [all data]

Meng, Griffiths, et al., 1996
Meng, L.J.; Griffiths, W.J.; Sjövall, J., The identification of novel steroid N-acetylglucosaminides in the urine of pregnant women, J. Steroid Biochem., 1996, 58, 5/6, 585-598, https://doi.org/10.1016/0960-0760(96)00080-5 . [all data]

Vandenheuvel, 1975
Vandenheuvel, F.A., Gas-liquid chromatographic studies of reactions and structural relationships of steroids, J. Chromatogr., 1975, 103, 1, 113-134, https://doi.org/10.1016/S0021-9673(00)83806-6 . [all data]


Notes

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