7-α-Hydroxycholesterol, TMS


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryUltra-13115.Axelson and Larsson, 199625. m/0.32 mm/0.17 μm; Program: 50C (3min) => (rapidly) => 185C => 5C/min => 280C
CapillaryOV-13104.Brooks, Cole, et al., 198325. m/0.25 mm/0.25 μm; Program: 80 0C (5 min) 40 K/min -> 120 0C (2 min) 2 K/min -> 200 0C (for androstanes), or 80 0C (5 min) 40 K/min -> 190 0C (2 min) 2 K/min -> 270 0C (for sterols)

References

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Axelson and Larsson, 1996
Axelson, M.; Larsson, O., 27-Hydroxylated low density lipoprotein (LDL) cholesterol can be converted to 7α,27-Dihydroxy-4-cholesten-3-one (cytosterone) before suppressing cholesterol production in normal human fibroblasts. Evidence that an altered metabolism of LDL cholesterol can underlie a defective feedback control in malignant cells, J. Biol. Chem., 1996, 271, 22, 12724-12736. [all data]

Brooks, Cole, et al., 1983
Brooks, C.J.W.; Cole, W.J.; Lawrie, T.D.V.; MacLachlan, J.; Borthwick, J.H.; Barrett, G.M., Selective reactions in the analytical characterisation of steroids by gas chromatography-mass spectrometry, J. Steroid Biochem., 1983, 19, 1, 189-201, https://doi.org/10.1016/S0022-4731(83)80025-9 . [all data]


Notes

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