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Kovats' RI, non-polar column, custom temperature program

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase HP-5DB-5MSHP-5DB-5HP-5
Column length (m)
Carrier gas HeHeHeHeHe
Column diameter (mm)
Phase thickness (mum)
Program 60C(5min) => 3C/min => 100C => 1C/min => 160C => 10C/min => 240C(10min)60C(5min) => 2C/min => 140C= 5C/min => 250C(5min)40C(10min) => 3C/min => 200C => 2C/min => 220C35C => 4C/min => 180C => 20C/min => 280Cnot specified
I 1481.1474.1476.1472.1477.
Referencede Marchese, de Heluani, et al., 2007Tuberoso, Kowalczyk, et al., 2005Sibanda, Chigwada, et al., 2004Araújo, Silveira, et al., 2003Shellie and Marriott, 2003
Column type CapillaryCapillaryCapillaryCapillaryCapillary
Active phase OV-1HP-5DB-5DB-5MSDB-5
Column length (m)
Carrier gas HeH2H2HeHe
Column diameter (mm) 0.250.320.200.250.25
Phase thickness (mum) 0.250.25 0.25 
Program 44C(4min) => 3C/min => 74C => 6C/min => 134C => 12C/min => 312C60 0C (8 min) 3 K/min -> 180 0C 20 K/min (20 min)not specified40C => 2C/min => 60C => 4C/min => 260Cnot specified
I 1478.1475.1477.1475.1491.
ReferenceEl-Shazly, Dorai, et al., 2002Frizzo, Serafini, et al., 2001Moreira, Sousa, et al., 2001Maia, Andrade, et al., 2000Rao, Rout, et al., 2000
Column type CapillaryCapillaryCapillary
Active phase DB-5DB-5Methyl Silicone
Column length (m) 30.30.25.
Carrier gas    
Column diameter (mm) 0.260.25 
Phase thickness (mum) 0.250.25 
Program not specified50C(5min) => 2C/min => 250C (60min) => 2C/min => 270C => 1C/min => 290C50 0C 4 0C/min -> 180 0C 10 0C/min -> 250 0C
I 1477.1463.1474.
ReferenceAdams, 1998Roscigno, 1998Machado, Militao, et al., 1995


Go To: Top, Kovats' RI, non-polar column, custom temperature program, Notes

Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

de Marchese, de Heluani, et al., 2007
de Marchese, M.J.A.; de Heluani, C.S.; Catalán, C.A.N.; Griffin, C.A.; Vaughn, J.B., Jr.; Herz, W., Incisol, an alcohol with a novel sesquiterpene skeleton from Xenophyllum incisum, Biochem. Syst. Ecol., 2007, 35, 3, 169-175, https://doi.org/10.1016/j.bse.2006.10.006 . [all data]

Tuberoso, Kowalczyk, et al., 2005
Tuberoso, C.I.G.; Kowalczyk, A.; Coroneo, V.; Russo, M.T.; Dessì, S.; Cabras, P., Chemical Composition and Antioxidant, Antimicrobial, and Antifungal Activities of the Essential Oil of Achillea ligustica All., J. Agric. Food Chem., 2005, 53, 26, 10148-10153, https://doi.org/10.1021/jf0518913 . [all data]

Sibanda, Chigwada, et al., 2004
Sibanda, S.; Chigwada, G.; Poole, M.; Gwebu, E.T.; Noletto, J.A.; Schmidt, J.M.; Rea, A.I.; Setzer, W.N., Composition and bioactivity of the leaf essential oil of Heteropyxis dehniae from Zimbabwe, J. Ethnopharmacol., 2004, 92, 1, 107-111, https://doi.org/10.1016/j.jep.2004.02.010 . [all data]

Araújo, Silveira, et al., 2003
Araújo, E.C.C.; Silveira, E.R.; Lima, M.A.S.; Neto, M.A.; de Andrade, I.L.; Lima, M.A.A.; Santiago, G.M.P.; Mesquita, A.L.M., Insecticidal activity and chemical composition of volatile oils from Hyptis martiusii Benth, J. Agric. Food Chem., 2003, 51, 13, 3760-3762, https://doi.org/10.1021/jf021074s . [all data]

Shellie and Marriott, 2003
Shellie, R.A.; Marriott, P.J., Comprehensive two-dimensional gas chromatography-mass spectrometry analysis of Pelargonium graveolens essential oil using rapid scanning quadrupole mass spectrometry, Analyst, 2003, 128, 7, 879-883, https://doi.org/10.1039/b304371a . [all data]

El-Shazly, Dorai, et al., 2002
El-Shazly, A.; Dorai, G.; Wink, M., Composition and antimicrobial activity of essential oil and hexane-ether extract of Tanacetum santolinoides (DC.) Feinbr. and Fertig, Z. Naturforsch., 2002, 57c, 620-623. [all data]

Frizzo, Serafini, et al., 2001
Frizzo, C.D.; Serafini, L.A.; Dellacassa, E.; Lorenzo, D.; Moyna, P., Essential oil of Baccharis uncinella DC. from Southern Brazil, Flavour Fragr. J., 2001, 16, 4, 286-288, https://doi.org/10.1002/ffj.998 . [all data]

Moreira, Sousa, et al., 2001
Moreira, D.L.; Sousa, P.O.; Pereira, N.A.; Cardoso, G.L., Effect of leaf essential oil from Piper solmsianum C.DC. in mice behaviour, An. Acad. Bras. Cienc., 2001, 73, 1, 1-7, https://doi.org/10.1590/S0001-37652001000100004 . [all data]

Maia, Andrade, et al., 2000
Maia, J.G.S.; Andrade, E.H.A.; Zoghbi, M.G.B., Volatile constituents of the leaves, fruits and flowers of cashew ( Anacardium occidentaleL.), J. Food Comp. Anal., 2000, 13, 3, 227-232, https://doi.org/10.1006/jfca.2000.0894 . [all data]

Rao, Rout, et al., 2000
Rao, Y.R.; Rout, P.K.; Jena, K.S.; Sahu, S.B., Composition of essential oil of citronella (Cymbopogon winterianus jowitt) grown in coastal Orissa, Fafai J., 2000, 2, 4, 29-31. [all data]

Adams, 1998
Adams, R.P., The essential oils and chemotaxonomy of Juniperus sect. Juniperus, Biochem. Syst. Ecol., 1998, 26, 6, 637-645, https://doi.org/10.1016/S0305-1978(98)00020-9 . [all data]

Roscigno, 1998
Roscigno, G., Estrazione supercritica di principi farmaceutici e biopesticidi, Tesi di Laurea, Universita degli Studi di Salerno, Salerno, Italy, 1998, 121. [all data]

Machado, Militao, et al., 1995
Machado, S.M.F.; Militao, J.S.L.T.; Facundo, V.A.; Ribeiro, A.; de Morais, S.M.; de Alencar, J.W.; Filho, R.B., Essential Oil of Conyza sumatrensis (Retz) Walk, J. Essent. Oil Res., 1995, 7, 1, 83-84, https://doi.org/10.1080/10412905.1995.9698470 . [all data]


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