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Pregnane-3,20-diol, (3«alpha»,5«beta»,20S)-, 2TMS derivative


Gas Chromatography

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Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Data compiled by: NIST Mass Spectrometry Data Center, William E. Wallace, director

Kovats' RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
PackedOV-1225.2744.Voelter, Jung, et al., 1971N2, GasChrom Q

Van Den Dool and Kratz RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryDB-12763.Ludwig-Koehn and Henning, 198630. m/0.25 mm/0.25 «mu»m, H2, 2. K/min; Tstart: 120. C; Tend: 280. C
PackedOV-1012780.Hurter and van Ree, 1979Gas-Chrom P (80-100 mesh), 1. K/min; Column length: 3.6 m; Tstart: 180. C
PackedSE-302760.Hurter and van Ree, 1979Gas-Chrom P (80-100 mesh), 1. K/min; Column length: 3.6 m; Tstart: 180. C

Van Den Dool and Kratz RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryVF-5MS2803.9Tret'yakov, 200730. m/0.25 mm/0.25 «mu»m, He; Program: Multi-step temperature program; T(initial)=60C; T(final)=270C

Normal alkane RI, non-polar column, isothermal

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Column type Active phase Temperature (C) I Reference Comment
CapillaryOV-101240.2794.Krupcik, Rutten, et al., 1976Column length: 30. m; Column diameter: 0.25 mm
CapillaryOV-101251.2806.Krupcik, Rutten, et al., 1976Column length: 30. m; Column diameter: 0.25 mm

Normal alkane RI, non-polar column, temperature ramp

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Column type Active phase I Reference Comment
CapillaryOV-1012786.Pfeifer and Spiteller, 1981H2, 2. K/min; Column length: 50. m; Column diameter: 0.30 mm; Tstart: 150. C; Tend: 300. C

Normal alkane RI, non-polar column, custom temperature program

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Column type Active phase I Reference Comment
CapillaryCross-Linked Methylsilicone2786.Meng, Griffiths, et al., 199625. m/0.32 mm/0.32 «mu»m; Program: 60C => 30C/min => 210C (1.5min) => 2.5C/min => 320C (20min)
CapillaryCross-Linked Methylsilicone2787.Meng, Griffiths, et al., 199625. m/0.32 mm/0.32 «mu»m; Program: 60C => 30C/min => 210C (1.5min) => 2.5C/min => 320C (20min)

References

Go To: Top, Gas Chromatography, Notes

Data compilation copyright by the U.S. Secretary of Commerce on behalf of the U.S.A. All rights reserved.

Voelter, Jung, et al., 1971
Voelter, W.; Jung, G.; Breitmaier, E.; Bouchon, G.; Bayer, E.; Gupta, D., Comparison of the trifluoroacetyl and trimethylsilylether derivaties for gas chromatography of steroids, Anal. Chim. Acta., 1971, 53, 1, 185-188, https://doi.org/10.1016/S0003-2670(01)80085-4 . [all data]

Ludwig-Koehn and Henning, 1986
Ludwig-Koehn, H.; Henning, H.V., Some aspects of quantification of neutral steroid metabolites in body fluids of healthy and uremic subjects by capillary gas chromatography, J. Hi. Res. Chromatogr. Chromatogr. Comm., 1986, 9, 1, 35-38, https://doi.org/10.1002/jhrc.1240090106 . [all data]

Hurter and van Ree, 1979
Hurter, P.; van Ree, T., Programmed-Temperature Gas Chromatography on Mixed Phases. Separation of Steroid Derivatives on Binary Mixtures, J. Chromatogr., 1979, 169, 93-100, https://doi.org/10.1016/0021-9673(75)85034-5 . [all data]

Tret'yakov, 2007
Tret'yakov, K.V., Retention Data. NIST Mass Spectrometry Data Center., NIST Mass Spectrometry Data Center, 2007. [all data]

Krupcik, Rutten, et al., 1976
Krupcik, J.; Rutten, G.A.F.M.; Rijks, J.A., The influence of temperature on the separation and characterization of steroids on non-polar glass capillary columns, Chem. zvesti, 1976, 30, 4, 469-479. [all data]

Pfeifer and Spiteller, 1981
Pfeifer, P.; Spiteller, G., Steroid Profiles of Healthy Individuals, J. Chromatogr., 1981, 223, 1, 21-32, https://doi.org/10.1016/S0378-4347(00)80064-X . [all data]

Meng, Griffiths, et al., 1996
Meng, L.J.; Griffiths, W.J.; Sjövall, J., The identification of novel steroid N-acetylglucosaminides in the urine of pregnant women, J. Steroid Biochem., 1996, 58, 5/6, 585-598, https://doi.org/10.1016/0960-0760(96)00080-5 . [all data]


Notes

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